Open AccessRegulation of pathogenesis-related protein-1a gene expression in tobacco.
Author(s) -
Scott Uknes,
Sandra Dincher,
Leslie Friedrich,
David Negrotto,
Shericca Williams,
Hope Thompson-Taylor,
Sharon Potter,
Eric Ward,
John Ryals
Publication year - 1993
Publication title -
the plant cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.324
H-Index - 341
eISSN - 1532-298X
pISSN - 1040-4651
DOI - 10.1105/tpc.5.2.159
Subject(s) - biology , cycloheximide , reporter gene , ectopic expression , gene expression , gene , salicylic acid , transcription (linguistics) , protein biosynthesis , regulation of gene expression , microbiology and biotechnology , genetics , linguistics , philosophy
Pathogenesis-related protein-1a (PR-1a) is a protein of unknown function that is strongly induced during the onset of systemic acquired resistance (SAR) in tobacco. The expression of PR-1a is under complex regulation that is controlled at least partially by the rate of transcription. In this study, we demonstrated that 661 bp of 5' flanking DNA was sufficient to impart tobacco mosaic virus and salicylic acid inducibility to a reporter gene. The PR-1a promoter did not respond significantly to treatments with either auxin or cytokinin. Experiments with the protein synthesis inhibitor cycloheximide indicated that protein synthesis is required for salicylate-dependent mRNA accumulation. At flowering, the PR-1a gene was expressed primarily in the mesophyll and epidermal tissues of the leaf blade and the sepals of the flower. Several artifacts, most importantly ectopic expression in pollen, were associated with the use of the beta-glucuronidase reporter gene.