Arabidopsis GLABROUS1 Gene Requires Downstream Sequences for Function. | Zendy

John C. Larkin | Zendy; David G Oppenheimer | Zendy; Susan Pollock | Zendy; M. David Marks | Zendy

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Arabidopsis GLABROUS1 Gene Requires Downstream Sequences for Function.

Author(s) -

John C. Larkin,

David G Oppenheimer,

Susan Pollock,

M. David Marks

Publication year - 1993

Publication title -

the plant cell

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.324

H-Index - 341

eISSN - 1532-298X

pISSN - 1040-4651

DOI - 10.1105/tpc.5.12.1739

Subject(s) - biology , enhancer , stipule , arabidopsis , reporter gene , gene , gus reporter system , genetics , myb , enhancer trap , gene expression , mutant , botany

The Arabidopsis GLABROUS1 (GL1) gene is a myb gene homolog required for the initiation of trichome development. In situ hybridization revealed that the highest levels of GL1 transcripts were present in developing trichomes. In contrast, previous work had shown that putative promoter sequences from the 5[prime] noncoding region of the GL1 gene directed the expression of a [beta]-glucuronidase (GUS) reporter gene only in stipules. Deletion analysis of the 3[prime] noncoding region of GL1 has identified an enhancer that is essential for GL1 function. Sequences from the region containing the enhancer, in conjunction with GL1 upstream sequences, direct the expression of a GUS reporter gene in leaf primordia and developing trichomes in addition to stipules, indicating that the downstream enhancer is required for the normal expression pattern of GL1.

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