Pumpkin phloem lectin genes are specifically expressed in companion cells. | Zendy

Dwight E. Bostwick | Zendy; Joanne M. Dannenhoffer | Zendy; Megan I. Skaggs | Zendy; R. M. Lister | Zendy; Brian A. Larkins | Zendy; Gary A. Thompson | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Pumpkin phloem lectin genes are specifically expressed in companion cells.

Author(s) -

Dwight E. Bostwick,

Joanne M. Dannenhoffer,

Megan I. Skaggs,

R. M. Lister,

Brian A. Larkins,

Gary A. Thompson

Publication year - 1992

Publication title -

the plant cell

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.324

H-Index - 341

eISSN - 1532-298X

pISSN - 1040-4651

DOI - 10.1105/tpc.4.12.1539

Subject(s) - phloem , biology , exudate , microbiology and biotechnology , lectin , biochemistry , polyclonal antibodies , gene , messenger rna , botany , genetics , antibody

Pumpkin phloem exudate contains two abundant phloem proteins: PP1 is a 96-kD protein that forms polymeric filaments in vivo, and PP2 is a 48-kD dimeric lectin. Polyclonal antibodies raised against pumpkin phloem exudate were used to isolate several cDNAs corresponding to PP1 and PP2. RNA gel blot analysis indicated that PP1 is encoded by an mRNA of approximately 2500 nucleotides, whereas PP2 subunits are encoded by an mRNA of 1000 nucleotides. Sequence analysis of PP2 cDNAs revealed a 654-bp open reading frame encoding a 218-amino acid polypeptide; this polypeptide had the carbohydrate binding characteristics of a PP2 subunit. The PP2 mRNA was localized within the phloem of pumpkin hypocotyl cross-sections based on in situ hybridization of a digoxigenin-labeled antisense probe. PP2 mRNA was found within the companion cells in both the bicollateral vascular bundles and the extrafascicular phloem network.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research