A Tripartite Interaction among the Basidiomycete Rhodotorula mucilaginosa, N2-Fixing Endobacteria, and Rice Improves Plant Nitrogen Nutrition | Zendy

Karnelia Paul | Zendy; Chinmay Saha | Zendy; Mayurakshi Nag | Zendy; Drishti Mandal | Zendy; Haraprasad Naiya | Zendy; Diya Sen | Zendy; Souvik Mitra | Zendy; Mohit Kumar | Zendy; Dipayan Bose | Zendy; G. N. Mukherjee | Zendy; Nabanita Naskar | Zendy; Susanta Lahiri | Zendy; Upal Das Ghosh | Zendy; Sudipta Tripathi | Zendy; Mousumi Poddar Sarkar | Zendy; Manidipa Banerjee | Zendy; Aleysia Kleinert | Zendy; Alex J. Valentine | Zendy; Sucheta Tripathy | Zendy; Senjuti Sinharoy | Zendy; A. Seal | Zendy

Open Access

A Tripartite Interaction among the Basidiomycete Rhodotorula mucilaginosa, N₂-Fixing Endobacteria, and Rice Improves Plant Nitrogen Nutrition

Author(s) -

Karnelia Paul,

Chinmay Saha,

Mayurakshi Nag,

Drishti Mandal,

Haraprasad Naiya,

Diya Sen,

Souvik Mitra,

Mohit Kumar,

Dipayan Bose,

G. N. Mukherjee,

Nabanita Naskar,

Susanta Lahiri,

Upal Das Ghosh,

Sudipta Tripathi,

Mousumi Poddar Sarkar,

Manidipa Banerjee,

Aleysia Kleinert,

Alex J. Valentine,

Sucheta Tripathy,

Senjuti Sinharoy,

A. Seal

Publication year - 2019

Publication title -

the plant cell

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.324

H-Index - 341

eISSN - 1532-298X

pISSN - 1040-4651

DOI - 10.1105/tpc.19.00385

Subject(s) - pseudomonas stutzeri , biology , oryza sativa , diazotroph , oryza , botany , ammonium , nitrogen fixation , biochemistry , gene , chemistry , bacteria , genetics , organic chemistry

Nitrogen (N) limits crop yield, and improvement of N nutrition remains a key goal for crop research; one approach to improve N nutrition is identifying plant-interacting, N 2 -fixing microbes. Rhodotorula mucilaginosa JGTA-S1 is a basidiomycetous yeast endophyte of narrowleaf cattail ( Typha angustifolia ). JGTA-S1 could not convert nitrate or nitrite to ammonium but harbors diazotrophic (N 2 -fixing) endobacteria ( Pseudomonas stutzeri ) that allow JGTA-S1 to fix N 2 and grow in a N-free environment; moreover, P. stutzeri dinitrogen reductase was transcribed in JGTA-S1 even under adequate N. Endobacteria-deficient JGTA-S1 had reduced fitness, which was restored by reintroducing P. stutzeri JGTA-S1 colonizes rice ( Oryza sativa ), significantly improving its growth, N content, and relative N-use efficiency. Endofungal P. stutzeri plays a significant role in increasing the biomass and ammonium content of rice treated with JGTA-S1; also, JGTA-S1 has better N 2 -fixing ability than free-living P. stutzeri and provides fixed N to the plant. Genes involved in N metabolism, N transporters, and NODULE INCEPTION -like transcription factors were upregulated in rice roots within 24 h of JGTA-S1 treatment. In association with rice, JGTA-S1 has a filamentous phase and P. stutzeri only penetrated filamentous JGTA-S1. Together, these results demonstrate an interkingdom interaction that improves rice N nutrition.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research