The RING Finger Ubiquitin E3 Ligase SDIR1 Targets SDIR1-INTERACTING PROTEIN1 for Degradation to Modulate the Salt Stress Response and ABA Signaling inArabidopsis | Zendy

Huawei Zhang | Zendy; Feng Cui | Zendy; Yaorong Wu | Zendy; Lijuan Lou | Zendy; Lijing Liu | Zendy; Miaomiao Tian | Zendy; Yuese Ning | Zendy; Kai Shu | Zendy; Sanyuan Tang | Zendy; Qi Xie | Zendy

Open Access

The RING Finger Ubiquitin E3 Ligase SDIR1 Targets SDIR1-INTERACTING PROTEIN1 for Degradation to Modulate the Salt Stress Response and ABA Signaling inArabidopsis

Author(s) -

Huawei Zhang,

Feng Cui,

Yaorong Wu,

Lijuan Lou,

Lijing Liu,

Miaomiao Tian,

Yuese Ning,

Kai Shu,

Sanyuan Tang,

Qi Xie

Publication year - 2015

Publication title -

the plant cell

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.324

H-Index - 341

eISSN - 1532-298X

pISSN - 1040-4651

DOI - 10.1105/tpc.114.134163

Subject(s) - ubiquitin ligase , abscisic acid , arabidopsis , biology , microbiology and biotechnology , ubiquitin , endoplasmic reticulum , proteasome , transcription factor , arabidopsis thaliana , signal transduction , unfolded protein response , ring finger , cop9 signalosome , biochemistry , gene , mutant , enzyme , protease , peptide hydrolases

The plant hormone abscisic acid (ABA) regulates many aspects of plant development and the stress response. The intracellular E3 ligase SDIR1 (SALT- AND DROUGHT-INDUCED REALLY INTERESTING NEW GENE FINGER1) plays a key role in ABA signaling, regulating ABA-related seed germination and the stress response. In this study, we found that SDIR1 is localized on the endoplasmic reticulum membrane in Arabidopsis thaliana. Using cell biology, molecular biology, and biochemistry approaches, we demonstrated that SDIR1 interacts with and ubiquitinates its substrate, SDIRIP1 (SDIR1-INTERACTING PROTEIN1), to modulate SDIRIP1 stability through the 26S proteasome pathway. SDIRIP1 acts genetically downstream of SDIR1 in ABA and salt stress signaling. In detail, SDIRIP1 selectively regulates the expression of the downstream basic region/leucine zipper motif transcription factor gene ABA-INSENSITIVE5, rather than ABA-RESPONSIVE ELEMENTS BINDING FACTOR3 (ABF3) or ABF4, to regulate ABA-mediated seed germination and the plant salt response. Overall, the SDIR1/SDIRIP1 complex plays a vital role in ABA signaling through the ubiquitination pathway.

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