Open AccessRestriction Fragment Length Polymorphism Linkage Map of Arabidopsis thaliana.
Author(s) -
Hong Gil Nam,
J. Giraudat,
Bart den Boer,
Francis Moonan,
WDB. Loos,
Brian M. Hauge,
H. M. Goodman
Publication year - 1989
Publication title -
the plant cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.324
H-Index - 341
eISSN - 1532-298X
pISSN - 1040-4651
DOI - 10.1105/tpc.1.7.699
Subject(s) - restriction fragment length polymorphism , biology , genetics , gene mapping , genome , arabidopsis , genetic linkage , amplified fragment length polymorphism , cosmid , restriction fragment , arabidopsis thaliana , genetic marker , restriction map , gene , genotype , chromosome , population , genetic diversity , mutant , plasmid , demography , sociology
We have constructed a restriction fragment length polymorphism (RFLP) linkage map of the nuclear genome of the small flowering plant Arabidopsis thaliana. The map is based on the meiotic segregation of both RFLP and morphological genetic markers from five independent crosses. The morphological markers on each of the five chromosomes were included in the crosses to allow alignment of the RFLP map with the established genetic map. The map contains 94 new randomly distributed molecular markers (nine identified cloned Arabidopsis genes and 85 genomic cosmid clones) that detect polymorphisms between the Landsberg erecta and Columbia races. In addition, 17 markers from an independently constructed RFLP map of the Arabidopsis genome [Chang, C., Bowman, J.L., DeJohn, A.W., Lander, E.S., and Meyerowitz, E.M. (1988). Proc. Natl. Acad. Sci. USA 85, 6856-6860] have been included to permit integration of the two RFLP maps.
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