Mapping of ribosomal RNA transcripts in wheat. | Zendy

Michel Vincentz | Zendy; R. B. Flavell | Zendy

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Mapping of ribosomal RNA transcripts in wheat.

Author(s) -

Michel Vincentz,

R. B. Flavell

Publication year - 1989

Publication title -

the plant cell

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.324

H-Index - 341

eISSN - 1532-298X

pISSN - 1040-4651

DOI - 10.1105/tpc.1.6.579

Subject(s) - biology , rna , primer extension , genetics , intergenic region , ribosomal rna , intron , transcription (linguistics) , base pair , spacer dna , 18s ribosomal rna , ribosomal intergenic spacer analysis , microbiology and biotechnology , dna , gene , internal transcribed spacer , genome , linguistics , philosophy

Ribosomal RNA transcripts in wheat have been studied by RNA gel blotting and their termini determined from electrophoretic analysis of S1 nuclease-resistant RNA/DNA hybrids and also of hybrids created by primer extension. A major putative transcription initiation site has been localized 1132 base pairs upstream from the 5' end of the 18S RNA sequence. A major putative processing site occurs 640 base pairs from the 5' end of the 18S RNA. Transcripts extending at least 750 base pairs beyond the 3' end of the 25S rRNA sequence into the array of intergenic repeats are present, as are transcripts covering the principal initiation sequence. This suggests that some transcripts extend through the intergenic DNA from one repeat unit into the next.

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