Constitutive expression of pathogenesis-related proteins PR-1, GRP, and PR-S in tobacco has no effect on virus infection. | Zendy

Huub J. M. Linthorst | Zendy; Ralph Meuwissen | Zendy; Serge Kauffmann | Zendy; John F. Bol | Zendy

Open Access

Constitutive expression of pathogenesis-related proteins PR-1, GRP, and PR-S in tobacco has no effect on virus infection.

Author(s) -

Huub J. M. Linthorst,

Ralph Meuwissen,

Serge Kauffmann,

John F. Bol

Publication year - 1989

Publication title -

the plant cell

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.324

H-Index - 341

eISSN - 1532-298X

pISSN - 1040-4651

DOI - 10.1105/tpc.1.3.285

Subject(s) - biology , tobacco mosaic virus , cauliflower mosaic virus , gene , gene expression , virus , complementary dna , chimeric gene , virology , pathogenesis related protein , tobamovirus , rna , microbiology and biotechnology , genetics , genetically modified crops , transgene

Samsun NN tobacco cells were transformed with chimeric genes for pathogenesis-related (PR) proteins derived from genomic (PR-1a, GRP) or cDNA (PR-S) clones under the transcriptional control of the cauliflower mosaic virus 35S promoter. Regenerated plants were assayed by RNA and protein gel blotting, and plants showing high specific expression of the inserted genes were selected for self-pollination and seed formation. Inspection of second generation transformants showed that constitutive expression of PR-1a, GRP, and PR-S in tobacco in general does not have an effect on the phenotypic appearance of the plants or the expression of other endogenous PR genes. Furthermore, constitutive expression of the above genes does not affect the susceptibility of the plants to infection with tobacco mosaic virus or alfalfa mosaic virus.

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