A Gene Encoding an Acyl Hydrolase Is Involved in Leaf Senescence in Arabidopsis | Zendy

Yuehui He | Zendy; Susheng Gan | Zendy

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A Gene Encoding an Acyl Hydrolase Is Involved in Leaf Senescence in Arabidopsis

Author(s) -

Yuehui He,

Susheng Gan

Publication year - 2002

Publication title -

the plant cell

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.324

H-Index - 341

eISSN - 1532-298X

pISSN - 1040-4651

DOI - 10.1105/tpc.010422

Subject(s) - arabidopsis , senescence , biology , gene , transgene , rna interference , rna , microbiology and biotechnology , genetics , mutant

SAG101, a leaf senescence-associated gene, was cloned from an Arabidopsis leaf senescence enhancer trap line and functionally characterized. Reporter gene and RNA gel blot analyses revealed that SAG101 was not expressed until the onset of senescence in leaves. A recombinant SAG101 fusion protein overexpressed in Escherichia coli displayed acyl hydrolase activity. Antisense RNA interference in transgenic plants delayed the onset of leaf senescence for approximately 4 days. Chemically induced overexpression of SAG101 caused precocious senescence in both attached and detached leaves of transgenic Arabidopsis plants. These data suggest that SAG101 plays a significant role in leaf senescence.

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