Identification and Characterization of GONST1, a Golgi-Localized GDP-Mannose Transporter in Arabidopsis | Zendy

Timothy C. Baldwin | Zendy; Michael Handford | Zendy; María-Isabel Yuseff | Zendy; Ariel Orellana | Zendy; Paul Dupree | Zendy

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Identification and Characterization of GONST1, a Golgi-Localized GDP-Mannose Transporter in Arabidopsis

Author(s) -

Timothy C. Baldwin,

Michael Handford,

María-Isabel Yuseff,

Ariel Orellana,

Paul Dupree

Publication year - 2001

Publication title -

the plant cell

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.324

H-Index - 341

eISSN - 1532-298X

pISSN - 1040-4651

DOI - 10.1105/tpc.010247

Subject(s) - golgi apparatus , arabidopsis , biology , nucleotide sugar , mannose , arabidopsis thaliana , copi , biochemistry , mutant , transporter , vacuole , microbiology and biotechnology , glucose transporter , gene , transport protein , nucleotide , secretory pathway , cell , cytoplasm , insulin , endocrinology

Transport of nucleotide sugars across the Golgi apparatus membrane is required for the luminal synthesis of a variety of plant cell surface components. We identified an Arabidopsis gene encoding a nucleotide sugar transporter (designated GONST1) that we have shown by transient gene expression to be localized to the Golgi. GONST1 complemented a GDP-mannose transport-defective yeast mutant (vrg4-2), and Golgi-rich vesicles from the complemented strain displayed increased GDP-mannose transport activity. GONST1 promoter::beta-glucuronidase studies suggested that this gene is expressed ubiquitously. The identification of a Golgi-localized nucleotide sugar transporter from plants will allow the study of the importance of this class of proteins in the synthesis of plant cell surface components such as cell wall polysaccharides.

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