Localized Patch Clamping of Plasma Membrane of a Polarized Plant Cell | Zendy

Alison R. Taylor | Zendy; Colin Brownlee | Zendy

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Localized Patch Clamping of Plasma Membrane of a Polarized Plant Cell

Author(s) -

Alison R. Taylor,

Colin Brownlee

Publication year - 1992

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.99.4.1686

Subject(s) - turgor pressure , rhizoid , cell wall , biophysics , membrane , cytoplasm , plasma , apex (geometry) , cell membrane , cell , clamping , chemistry , microbiology and biotechnology , biology , botany , biochemistry , physics , quantum mechanics , engineering , mechanical engineering

We used an ultraviolet laser to rupture a small region of cell wall of a polarized Fucus spiralis rhizoid cell and gained localized access to the plasma membrane at the growing apex. Careful control of cell turgor enabled a small portion of plasma membrane-bound cytoplasm to be exposed. Gigaohm seals allowing single-channel recordings were obtained with a high success rate using this method with conventional patch clamp techniques.

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