Immunological Detection of Acetaldehyde-Protein Adducts in Ethanol-Treated Carrot Cells | Zendy

Pierdomenico Perata | Zendy; Paolo Vernieri | Zendy; D. Armellini | Zendy; M Bugnoli | Zendy; F. Togi | Zendy; Amedeo Alpi | Zendy

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Immunological Detection of Acetaldehyde-Protein Adducts in Ethanol-Treated Carrot Cells

Author(s) -

Pierdomenico Perata,

Paolo Vernieri,

D. Armellini,

M Bugnoli,

F. Togi,

Amedeo Alpi

Publication year - 1992

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.98.3.913

Subject(s) - acetaldehyde , daucus carota , chemistry , sodium cyanoborohydride , ethanol , biochemistry , propionaldehyde , adduct , conjugate , polyclonal antibodies , moiety , organic chemistry , antibody , biology , mathematical analysis , botany , mathematics , immunology

Polyclonal antibodies able to recognize protein-acetaldehyde conjugates were produced and characterized. The antibodies react with sodium cyanoborohydride-reduced Schiff's bases between acetaldehyde and a protein, independently of the nature of the macromolecule binding the acetaldehyde moiety. Only conjugates between acetaldehyde or propionaldehyde and a protein are recognized; conjugates obtained with other aldehydes are not reactive. Results concerning the formation of acetaldehyde adducts with carrot (Daucus carota L.) proteins are presented as well as the presence of such conjugates in ethanol-treated carrot cell cultures, a system highly sensitive to the presence of ethanol in the culture medium.

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