Hysteresis and Cooperative Behavior of a Latent Plant Polyphenoloxidase | Zendy

Edelmira Valero | Zendy; Francisco Garcı́a-Carmona | Zendy

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Hysteresis and Cooperative Behavior of a Latent Plant Polyphenoloxidase

Author(s) -

Edelmira Valero,

Francisco Garcı́a-Carmona

Publication year - 1992

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.98.2.774

Subject(s) - vitis vinifera , enzyme , chemistry , substrate (aquarium) , kinetics , lag , enzyme assay , hysteresis , lag time , biochemistry , biophysics , chromatography , botany , biology , biological system , ecology , computer network , physics , quantum mechanics , computer science

Appearance of a lag period dependent on pH in the expression of the catecholase activity of a polyphenoloxidase extracted in a latent state from Airen grape (Vitis vinifera L.) berries, is revealed, suggesting the hysteretic nature of the enzyme. The lag time was independent of enzyme concentration, indicating that slow pH-induced conformational changes in the protein must occur during assay. Results obtained by varying substrate concentration show that the system presents hyperbolic or cooperative kinetics depending on the pH of the assay.

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