High Performance Liquid Chromatography Analysis of Carbohydrates of Cotton-Phloem Sap and of Honeydew Produced by Bemisia tabaci Feeding on Cotton | Zendy

Mitchell C. Tarczynski | Zendy; David N. Byrne | Zendy; William B. Miller | Zendy

Open Access

High Performance Liquid Chromatography Analysis of Carbohydrates of Cotton-Phloem Sap and of Honeydew Produced by Bemisia tabaci Feeding on Cotton

Author(s) -

Mitchell C. Tarczynski,

David N. Byrne,

William B. Miller

Publication year - 1992

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.98.2.753

Subject(s) - honeydew , sucrose , phloem , fructose , exudate , whitefly , aphid , biology , carbohydrate , botany , malvaceae , gossypium hirsutum , sugar , chemistry , horticulture , food science , biochemistry

Phloem sap from cotton (Gossypium hirsutum L.) was collected from young and mature leaves by the aphid-stylet technique. Exudate was analyzed for carbohydrates by HPLC using sensitive pulsed amperometric detection. The predominant carbohydrate present (>90%) was identified as sucrose. A second, unidentified compound that was not one of the more commonly translocated sugars was detected in mature leaves. Carbohydrates in honeydew produced by the sweet-potato whitefly (Bemisia tabaci [Genn.]) feeding on cotton were sucrose, glucose, fructose, trehalulose, and a series of oligosaccharides.

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