Identification and Analysis of a Barley cDNA Clone Encoding the 31-Kilodalton LHC IIa (CP29) Apoprotein of the Light-Harvesting Antenna Complex of Photosystem II | Zendy

Daryl T. Morishige | Zendy; J. Philip Thornber | Zendy

Open Access

Identification and Analysis of a Barley cDNA Clone Encoding the 31-Kilodalton LHC IIa (CP29) Apoprotein of the Light-Harvesting Antenna Complex of Photosystem II

Author(s) -

Daryl T. Morishige,

J. Philip Thornber

Publication year - 1992

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.98.1.238

Subject(s) - hordeum vulgare , complementary dna , peptide sequence , biology , biochemistry , microbiology and biotechnology , gene , botany , poaceae

The light-harvesting complex (LHC) of photosystem II is composed of several different pigment-binding apoproteins. We have identified a cDNA clone LHCIIa-1 encoding the 31-kilodalton LHC IIa (CP29, Chl a/b-P1) apoprotein of barley (Hordeum vulgare). Direct protein microsequencing of an internal peptide fragment from the LHC IIa apoprotein has been used to identify unequivocally the cDNA clone as that coding for the LHC IIa apoprotein. Microsequencing of the 28-kilodalton LHC IIc protein (CP26) showed only minor sequence similarity to the LHC IIa protein, indicating that they are two different gene products. LHCIIa-1 codes for a protein of 286 amino acid residues (molecular weight, 31,308), which displays strong similarities to other pigment-binding LHC proteins, and yet contains an additional 42 amino acid residue segment. Two regions of strong intramolecular sequence similarity are also observed.

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