Open AccessEfficiency of Particle-Bombardment-Mediated Transformation Is Influenced by Cell Cycle Stage in Synchronized Cultured Cells of Tobacco
Author(s) -
Aritoshi Iida,
Toshiya Yamashita,
Yasuyuki Yamada,
Hiromichi Morikawa
Publication year - 1991
Publication title -
plant physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.554
H-Index - 312
eISSN - 1532-2548
pISSN - 0032-0889
DOI - 10.1104/pp.97.4.1585
Subject(s) - nicotiana tabacum , transformation (genetics) , particle (ecology) , cell cycle , plant cell , biology , microbiology and biotechnology , plasmid , gene , cell , chemistry , botany , genetics , ecology
Plasmid DNA pB1221 harboring beta-glucuronidase gene was delivered to synchronized cultured tobacco (Nicotiana tabacum L. cv Bright Yellow-2) cells of different cell cycle stages by a pneumatic particle gun. The cells bombarded at M and G(2) phases gave 4 to 6 times higher transformation efficiency than those bombarded at the S and G(1) phases.