Efficiency of Particle-Bombardment-Mediated Transformation Is Influenced by Cell Cycle Stage in Synchronized Cultured Cells of Tobacco | Zendy

Asako Iida | Zendy; Tadashi Yamashita | Zendy; Yasuyuki Yamada | Zendy; Hiromichi Morikawa | Zendy

Open Access

Efficiency of Particle-Bombardment-Mediated Transformation Is Influenced by Cell Cycle Stage in Synchronized Cultured Cells of Tobacco

Author(s) -

Asako Iida,

Tadashi Yamashita,

Yasuyuki Yamada,

Hiromichi Morikawa

Publication year - 1991

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.97.4.1585

Subject(s) - nicotiana tabacum , transformation (genetics) , particle (ecology) , cell cycle , plant cell , biology , microbiology and biotechnology , plasmid , gene , cell , chemistry , botany , genetics , ecology

Plasmid DNA pB1221 harboring beta-glucuronidase gene was delivered to synchronized cultured tobacco (Nicotiana tabacum L. cv Bright Yellow-2) cells of different cell cycle stages by a pneumatic particle gun. The cells bombarded at M and G(2) phases gave 4 to 6 times higher transformation efficiency than those bombarded at the S and G(1) phases.

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