Evidence That More than 90% of β-Glucuronidase-Expressing Cells after Particle Bombardment Directly Receive the Foreign Gene in their Nucleus | Zendy

Tadashi Yamashita | Zendy; Asako Iida | Zendy; Hiromichi Morikawa | Zendy

Open Access

Evidence That More than 90% of β-Glucuronidase-Expressing Cells after Particle Bombardment Directly Receive the Foreign Gene in their Nucleus

Author(s) -

Tadashi Yamashita,

Asako Iida,

Hiromichi Morikawa

Publication year - 1991

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.97.2.829

Subject(s) - nicotiana tabacum , gene gun , nucleus , glucuronidase , particle (ecology) , beta glucuronidase , gene , microbiology and biotechnology , dna , transfection , biology , intracellular , plant cell , chemistry , gene expression , plasmid , genetics , dna vaccination , ecology

Plasmid DNA harboring the beta-glucuronidase (GUS) gene, coated on gold particles, was delivered into cultured tobacco (Nicotiana tabacum L. cv Bright Yellow-2) cells using a pneumatic particle gun. Cytological analyses of intracellular location of the introduced gold particles before and after GUS expression assay indicated that more than 90% of GUS-expressing cells after bombardment received a DNA-coated particle in their nucleus.

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