Nucleotide Sequence of a Complementary DNA Encoding Pea Cytosolic Copper/Zinc Superoxide Dismutase | Zendy

D. A. White | Zendy; Barbara A. Zilinskas | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Nucleotide Sequence of a Complementary DNA Encoding Pea Cytosolic Copper/Zinc Superoxide Dismutase

Author(s) -

D. A. White,

Barbara A. Zilinskas

Publication year - 1991

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.96.4.1391

Subject(s) - nucleic acid sequence , biology , biochemistry , peptide sequence , complementary dna , open reading frame , amino acid , pisum , cytosol , nucleotide , microbiology and biotechnology , dna , gene , enzyme

The authors now report the nucleotide sequence of the cytosolic Cu/Zn SOD cloned from a {lambda}gt11 cDNA library constructed from mRNA extracted from leaves of 7- to 10-d pea seedlings (Pisum sativum L.). The clone was isolated using a 22-base synthetic oligonucleotide complementary to the amino acid sequence CGIIGLQG. This sequence, found at the protein's carboxy terminus, is highly conserved among plant cytosolic Cu/Zn SODs but not chloroplastic Cu/Zn SODs. The 738-base pair sequence contains an open reading frame specifying 152 codons and a predicted M{sub r} of 18,024 D. The deduced amino acid sequence is highly homologous (79-82% identity) with the sequences of other known plant cytosolic Cu/Zn SODs but less highly conserved (63-65%) when compared with several chloroplastic Cu/Zn SODs including pea (10).

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research