Evidence of Zein-Bound Indoleacetic Acid Using Gas Chromatography-Selected Ion Monitoring-Mass Spectrometry Analysis and Immunogold Labeling | Zendy

Leslie A. Leverone | Zendy; W. Kossenjans | Zendy; Koka Jayasimihulu | Zendy; John L. Caruso | Zendy

Open Access

Evidence of Zein-Bound Indoleacetic Acid Using Gas Chromatography-Selected Ion Monitoring-Mass Spectrometry Analysis and Immunogold Labeling

Author(s) -

Leslie A. Leverone,

W. Kossenjans,

Koka Jayasimihulu,

John L. Caruso

Publication year - 1991

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.96.4.1070

Subject(s) - immunogold labelling , chemistry , chromatography , mass spectrometry , isotope dilution , hydrolysis , gas chromatography–mass spectrometry , size exclusion chromatography , immunoelectron microscopy , mole , gas chromatography , biochemistry , botany , biology , enzyme , ultrastructure , immunohistochemistry , immunology

Commercial zein was base-hydrolyzed and purified extracts were subjected to gas chromatography-selected ion monitoring-mass spectrometry analysis. Indoleacetic acid (IAA) was shown to be released from this storage protein of corn (Zea mays). Isotope dilution using [(13)C(6)]IAA as an internal standard revealed a conservative ratio of 1 mole IAA to 175 moles zein. Immunoelectron micrographs of isolated protein bodies also showed IAA or an IAA-like molecule associated with zein and deposited within these organelles.

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