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We have used antibodies directed against the two sucrose synthase (SS) isozymes, and the cDNA clones corresponding to the two nonallelic genes in maize to describe sorghum (Sorghum bicolor) SS genes and their expressions at protein and RNA levels. Western blot analyses have shown evidence of two SS isozymes, SS1 and SS2, in sorghum; these were similar, but not identical, to maize isozymes in size, charge, subunit composition, and epitope specificities against both monoclonal and polyclonal antibodies. Tissue-specific distributions of isozymes and genomic Southern hybridization data are consistent with a hypothesis that the SS1 and SS2 isozymes are encoded by two nonallelic genes, designated here as Sus1 and Sus2, respectively. Northern blot hybridizations on root RNAs showed gene-specific transcript patterns and, as in maize, the SS2-specific transcripts were slightly larger than the SS1-specific transcripts. Interestingly, no difference in the size of the SS1 and SS2 polypeptides was detected. Anaerobic induction led to significant elevations in steady-state levels of both SS1 and SS2 transcripts, but there was no detectable increase in the levels of the SS proteins. Thus, both the SS genes in sorghum were significantly regulated at the posttranscriptional level; whereas in maize, only one of the two SS genes was affected in this fashion. Another difference between maize and sorghum SS isozymes was in endosperm-specific polymerization among the SS subunits. Unlike maize endosperm where only the two SS homotetramers are seen, sorghum endosperm showed five SS isozymes attributable to a random copolymerization of SS1 and SS2 subunits, presumably due to a simultaneous expression of both genes in the endosperm cells. Physiological and molecular bases of these differences between these two crop plant species remains to be elucidated.

Tissue-Specific Expression and Anaerobically Induced Posttranscriptional Modulation of Sucrose Synthase Genes in Sorghum bicolor M.