Sucrose Phosphate Synthase, a Key Enzyme for Sucrose Biosynthesis in Plants | Zendy

JeanMichel Bruneau | Zendy; Ann C. Worrell | Zendy; Bernard Cambou | Zendy; Danielle Lando | Zendy; Toni A. Voelker | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Sucrose Phosphate Synthase, a Key Enzyme for Sucrose Biosynthesis in Plants

Author(s) -

JeanMichel Bruneau,

Ann C. Worrell,

Bernard Cambou,

Danielle Lando,

Toni A. Voelker

Publication year - 1991

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.96.2.473

Subject(s) - sucrose phosphate synthase , enzyme , sucrose , biochemistry , specific activity , atp synthase , enzyme assay , biosynthesis , phosphate , zea mays , monoclonal antibody , molecular mass , chemistry , biology , sucrose synthase , antibody , agronomy , immunology , invertase

We have purified the protein for the enzyme sucrose phosphate synthase (SPS) from corn (Zea mays) leaves. Partially purified SPS protein was used to generate specific monoclonal antibodies. The following immunoaffinity chromatography allowed the isolation of pure SPS protein. The apparent molecular mass of the SPS polypeptide is 138 kilodaltons. By immunoblot, an SPS antigen was found to accumulate in mature leaves. SPS protein levels remain constant during the day/night cycle. The observed diurnal fluctuation of extractable enzyme activity, therefore, must be caused by modification of the specific activity of SPS in vivo.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research