Differential Expression of the Two Arabidopsis Nitrate Reductase Genes | Zendy

ChiLien Cheng | Zendy; Gregoria N. Acedo | Zendy; Julia Dewdney | Zendy; Howard M. Goodman | Zendy; Mark A. Conkling | Zendy

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Differential Expression of the Two Arabidopsis Nitrate Reductase Genes

Author(s) -

ChiLien Cheng,

Gregoria N. Acedo,

Julia Dewdney,

Howard M. Goodman,

Mark A. Conkling

Publication year - 1991

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.96.1.275

Subject(s) - nitrate reductase , arabidopsis , circadian rhythm , arabidopsis thaliana , biology , nitrate , gene , gene expression , mutant , botany , biochemistry , genetics , microbiology and biotechnology , endocrinology , ecology

The differential regulation of the two nitrate reductase (NR, EC 1.6.6.1) genes of Arabidopsis thaliana L. Heynh was examined. cDNAs corresponding to each of the NR genes (NR1 and NR2) were used to measure changes in the steady-state levels of NR mRNA in response to nitrate, light, circadian rhythm, and tissue specificity. Although nitrate-induction kinetics of the two genes are very similar, NR1 is expressed in the absence of nitrate at a higher basal level than NR2. Nitrate induction is transient both in the roots and leaves, however the kinetics are different: the induction and decline in the roots precede that in the leaves. Light induces the expression of each of the genes with significantly different kinetics: NR2 reached saturation more rapidly than did NR1. Both genes showed similar diurnal patterns of circadian rhythm, with NR2 mRNA accumulating earlier in the morning.

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