Conditions Favorable for the Somatic Embryogenesis in Carrot Cell Culture Enhance Expression of the roIC Promoter-GUS Fusion Gene | Zendy

Nobuharu Fujii | Zendy; Hirofumi Uchimiya | Zendy

Open Access

Conditions Favorable for the Somatic Embryogenesis in Carrot Cell Culture Enhance Expression of the roIC Promoter-GUS Fusion Gene

Author(s) -

Nobuharu Fujii,

Hirofumi Uchimiya

Publication year - 1991

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.95.1.238

Subject(s) - somatic embryogenesis , daucus carota , beta glucuronidase , agrobacterium , biology , transformation (genetics) , gene expression , microbiology and biotechnology , gene , somatic cell , embryo , abscisic acid , embryogenesis , glucuronidase , cell culture , biochemistry , genetics , botany

We obtained carrot (Daucus carota) cells possessing the 5'-noncoding sequence of the ORF12 gene (roIC) of TL-DNA of the Ri plasmid and a structural gene of bacterial beta-glucuronidase by Agrobacterium-mediated transformation. When such cells were cultured in medium containing 2,4-dichlorophenoxyacetic acid, substantial reduction in beta-glucuronidase activity was observed. Upon transferring the cells from a 2,4-D-containing medium to one devoid of 2,4-dichlorophenoxyacetic acid, enhanced expression of beta-glucuronidase in somatic embryo development was recorded. Activation by gibberillic acid and suppression by abscisic acid of beta-glucuronidase activities, in concord with embryogenesis, were also noted.

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