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Isolation and Characterization of a Novel Nuclear Protein from Pollen Mother Cells of Lily
Author(s) -
Yoh Sasaki,
Hideyo Yasuda,
Yoshiki Ohba,
Hiroshi Harada
Publication year - 1990
Publication title -
plant physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.554
H-Index - 312
eISSN - 1532-2548
pISSN - 0032-0889
DOI - 10.1104/pp.94.3.1467
Subject(s) - pollen , isolation (microbiology) , biology , botany , microbiology and biotechnology
Pollen mother cells of the lily (Lilium speciosum) were found to have a histone-H1-like protein (PMCP) not detected in other tissues. The PMCP appears from the late S-G(2) period of premeiosis and is present in mature pollen. PMCP and H1 were extracted from pollen mother cells with 5% perchloric acid and isolated by reverse-phase high-performance liquid chromatography. The amino acid composition of PMCP differs from that of somatic H1. However, PMCP is similar to H1t in mammalian testis with regard to amino acid composition.

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