Partial Purification and Characterization of the Gibberellin A20 3β-Hydroxylase from Seeds of Phaseolus vulgaris | Zendy

Valerie Smith | Zendy; Paul Gaskin | Zendy; Jake MacMillan | Zendy

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Partial Purification and Characterization of the Gibberellin A₂₀ 3β-Hydroxylase from Seeds of Phaseolus vulgaris

Author(s) -

Valerie Smith,

Paul Gaskin,

Jake MacMillan

Publication year - 1990

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.94.3.1390

Subject(s) - phaseolus , gibberellin , botany , characterization (materials science) , biology , materials science , nanotechnology

The GA(20) 3beta-hydroxylase present in immature seeds of Phaseolus vulgaris has been partially purified and characterized. The physical characteristics of the enzyme are similar to those of the GA 2beta-hydroxylases present in mature and immature seeds of Pisum sativum. It is acid-labile, hydrophobic, and of M(r) 45,000. The enzyme catalyzes the synthesis of GA(1), GA(5), and GA(29) from GA(20). Activity is dependent upon the presence of Fe(2+), ascorbate, 2-oxoglutarate, and oxygen. 2-Oxoglutarate does not function as a cosubstrate; in the presence of the enzyme, succinate is not a reaction product.

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