Purification and Characterization of a Type-I Topoisomerase from Cultured Tobacco Cells | Zendy

S. HeathPagliuso | Zendy; Allyson D. Cole | Zendy; Eric B. Kmiec | Zendy

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Purification and Characterization of a Type-I Topoisomerase from Cultured Tobacco Cells

Author(s) -

S. HeathPagliuso,

Allyson D. Cole,

Eric B. Kmiec

Publication year - 1990

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.94.2.599

Subject(s) - topoisomerase , nicotiana tabacum , chemistry , cell culture , biology , botany , biochemistry , dna , genetics , gene

Cultured tobacco (Nicotiana tabacum, var Xanthi) cells contain a topoisomerase that removes positive and negative supercoils from DNA. The enzyme has an estimated molecular mass of 30,000 daltons under denaturing conditions, but may exist as a multimeric protein in the native state. Activity is enhanced significantly by either MgCl(2) or CaCl(2), but other divalent cations are much less effective in stimulating DNA relaxation. The purified enzyme acts by altering the linking number in topological steps of one and is inhibited by berenil or camptothecin, not novobiocin. Taken together, these data identify this enzyme as a type I topoisomerase.

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