Thermal Dependence of the Apparent Km of Glutathione Reductases from Three Plant Species

The thermal dependencies of the apparent K(m) of the glutathione reductases from spinach (Spinacia oleracea L.) corn (Zea mays L.), and cucumber (Cucumis sativus L.) were determined. The apparent K(m) of the enzymes were found to vary up to 9-fold between 12.5 and 45 degrees C. Values of the apparent K(m) in excess of 200% of the observed minimum are suggested to be detrimental to the normal function of the enzyme. We propose the term "thermal kinetic window" to describe to the range of temperatures over which the apparent K(m) of the glutathione reductase is within 200% of its minimum and suggest that it may be a useful indicator of the limits of thermal stress for a given species. The thermal kinetic windows determined in this study are: <16 degrees C for spinach, 23 to 32 degrees C for corn, and 35 to 41 degrees C for cucumber.