Hordeum vulgare Seedlings Amine Oxidase | Zendy

Antonina Cogoni | Zendy; Carla Piras | Zendy; Raffaele Farci | Zendy; Antonello Melis | Zendy; Giovanni Floris | Zendy

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Hordeum vulgare Seedlings Amine Oxidase

Author(s) -

Antonina Cogoni,

Carla Piras,

Raffaele Farci,

Antonello Melis,

Giovanni Floris

Publication year - 1990

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.93.2.818

Subject(s) - hordeum vulgare , sephadex , chemistry , ammonium sulfate precipitation , ammonium , chromatography , cellulose , size exclusion chromatography , amine oxidase , phosphate , enzyme , hydroxylapatite , ammonium sulfate , biochemistry , poaceae , biology , botany , organic chemistry

Although no amine oxidase could be detected in crude extracts, the enzyme has been purified to apparent homogeneity from Hordeum vulgare seedlings using ammonium sulfate precipitation and chromatography on DEAE cellulose, Hydroxylapatite, and Sephadex G200 columns. Gel filtration experiments indicate a molecular weight of about 150,000. The pH optimum of the enzyme was found to be 7.5 in potassium phosphate buffer. The spectrum of ultraviolet and visible regions were similar to Cuamine oxidase from Leguminosae.

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