On The Regulation of Spinach Nitrate Reductase | Zendy

J. Pérez Sánchez | Zendy; Hans Walter Heldt | Zendy

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On The Regulation of Spinach Nitrate Reductase

Author(s) -

J. Pérez Sánchez,

Hans Walter Heldt

Publication year - 1990

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.92.3.684

Subject(s) - spinach , nitrate reductase , nitrate , biochemistry , nad+ kinase , malate dehydrogenase , reductase , chemistry , dehydrogenase , enzyme , spinacia , nucleotide , chloroplast , organic chemistry , gene

A coupled assay has been worked out to study spinach (Spinacea oleracea L.) nitrate reductase under low, more physiological concentrations of NADH. In this assay the reduction of nitrate is coupled to the oxidation of malate catalyzed by spinach NAD-malate dehydrogenase. The use of this coupled system allows the assay of nitrate reductase activity at steady-state concentrations of NADH below micromolar. We have used this coupled assay to study the kinetic parameters of spinach nitrate reductase and to reinvestigate the putative regulatory role of adenine nucleotides, inorganic phosphate, amino acids, and calcium and calmodulin.

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