Subcellular Compartmentation of Uridine Nucleotides and Nucleoside-5′ -Diphosphate Kinase in Leaves | Zendy

Jane E. Dancer | Zendy; H. Ekkehard Neuhaus | Zendy; Mark Stitt | Zendy

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Subcellular Compartmentation of Uridine Nucleotides and Nucleoside-5′ -Diphosphate Kinase in Leaves

Author(s) -

Jane E. Dancer,

H. Ekkehard Neuhaus,

Mark Stitt

Publication year - 1990

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.92.3.637

Subject(s) - spinacia , spinach , biochemistry , nucleotide , cytosol , nucleoside , differential centrifugation , uridine , nucleoside diphosphate kinase , biology , hordeum vulgare , uridine diphosphate , chenopodiaceae , kinase , chloroplast , enzyme , rna , botany , poaceae , gene

The subcellular compartmentation of nucleoside diphosphate kinase (EC 2.7.4.6) and the uridine nucleotides has been studied in leaves. Membrane filtration of barley (Hordeum vulgare L.) leaf mesophyll protoplasts and differential centrifugation of spinach (Spinacia oleracea L.) leaf extracts showed that about half the nucleoside diphosphate kinase is present in the cytosol. The activity is adequate to account for the turnover of UTP and UDP during photosynthetic sucrose synthesis. Nonaqueous density gradient centrifugation of freeze-stopped, lyophilized spinach leaf material showed that the uridine nucleotides are predominantly located in the cytosol and that the cytosolic UDP-glucose pool is considerably larger than the UTP or UDP pools.

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