In Vivo Biosynthetic Studies of the Dolichos biflorus Seed Lectin
Author(s) -
Jeanette M. Quinn,
Marilynn E. Etzler
Publication year - 1989
Publication title -
plant physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.554
H-Index - 312
eISSN - 1532-2548
pISSN - 0032-0889
DOI - 10.1104/pp.91.4.1382
Subject(s) - lectin , dolichos , in vivo , biology , botany , chemistry , biochemistry , microbiology and biotechnology
The in vivo biosynthesis of the Dolichos biflorus seed lectin was studied by pulse-chase labeling experiments using [(35)S] methionine and [(14)C]glucosamine. These studies demonstrate that each of the two mature lectin subunit types are derived by the processing of separate glycosylated precursors. The appearance of the precursor to subunit I before the precursor to subunit II supports the possibility raised by previous studies that both subunit types of this lectin may originate from a single gene product.
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