Transient Expression of Chimeric Genes Delivered into Pollen by Microprojectile Bombardment | Zendy

David Twell | Zendy; Theodore M. Klein | Zendy; Michael Fromm | Zendy; Susan P. McCormick | Zendy

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Transient Expression of Chimeric Genes Delivered into Pollen by Microprojectile Bombardment

Author(s) -

David Twell,

Theodore M. Klein,

Michael Fromm,

Susan P. McCormick

Publication year - 1989

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.91.4.1270

Subject(s) - nicotiana tabacum , pollen , lycopersicon , chimeric gene , biology , gene , transformation (genetics) , botany , nicotiana , solanaceae , gene expression , genetics

Chimeric genes containing a pollen-specific promoter from tomato (Lycopersicon esculentum) or the CaMV35S promoter were transiently expressed following their introduction into tobacco (Nicotiana tabacum) pollen using high velocity microprojectiles. Transient expression of the microprojectile-introduced genes in leaves and pollen was similar to that observed for these genes in stably transformed tobacco plants.

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