DNA Methylation is Reduced in DNasel-Sensitive Regions of Plant Chromatin | Zendy

Manfred Klaas | Zendy; Richard M. Amasino | Zendy

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DNA Methylation is Reduced in DNasel-Sensitive Regions of Plant Chromatin

Author(s) -

Manfred Klaas,

Richard M. Amasino

Publication year - 1989

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.91.2.451

Subject(s) - chromatin , dna methylation , dna , biology , methylation , biochemistry , fraction (chemistry) , microbiology and biotechnology , chemistry , gene expression , gene , chromatography

Pea, barley, and corn chromatin was isolated and subjected to limited digestion with DNasel. The preferentially degraded, small molecular weight fraction, presumably consisting of sequences in an active state of expression, was isolated and its 5-methylcytosine content determined. The DNasel-sensitive chromatin fraction from all three plant species investigated contained a markedly reduced level of DNA methylation compared to total DNA.

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