An Immunological Approach to Gibberellin Purification and Quantification | Zendy

Valerie Smith | Zendy; Jake MacMillan | Zendy

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An Immunological Approach to Gibberellin Purification and Quantification

Author(s) -

Valerie Smith,

Jake MacMillan

Publication year - 1989

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.90.3.1148

Subject(s) - gibberellin , affinity chromatography , chromatography , monoclonal antibody , gas chromatography , chemistry , radioimmunoassay , mass spectrometry , biology , biochemistry , antibody , botany , immunology , enzyme

Gibberellin (GA) specific, high-affinity monoclonal antibodies have been used to assay the GA content of various plant tissues and to purify selected GAs by immunoaffinity chromatography. These immunological techniques may not stand alone as a general method of GA analysis. The results of this study indicate, however, that in conjunction with gas chromatography-mass spectrometry for positive GA identification, radioimmunoassay and immunoaffinity chromatography are extremely powerful tools for purifying and quantifying GAs from plant tissues.

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