Open Accessδ13C Values in Maize Leaf Correlate with Phosphoenolpyruvate Carboxylase Levels
Author(s) -
Hideo Sasakawa,
Bambang Sugiharto,
Marion H. O’Leary,
Tatsuo Sugiyama
Publication year - 1989
Publication title -
plant physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.554
H-Index - 312
eISSN - 1532-2548
pISSN - 0032-0889
DOI - 10.1104/pp.90.2.582
Subject(s) - phosphoenolpyruvate carboxylase , pyruvate carboxylase , photosynthesis , rubisco , carboxylation , ribulose , oxygenase , carbon fixation , phosphoenolpyruvate carboxykinase , chemistry , biology , botany , biochemistry , enzyme , catalysis
Values of delta(13)C and levels of phosphoenolpyruvate carboxylase and ribulose 1,5-bisphosphate carboxylase/oxygenase were analyzed in segments from the fourth leaf of young maize (Zea mays L.) plants. The delta(13)C values became significantly more negative from the base to the tip of the leaves. Phosphoenolpyruvate carboxylase levels and ribulose bisphosphate carboxylase levels both increased from the base to the tip. The principal effect of phosphoenolpyruvate carboxylase levels or delta(13)C should arise through its effect on the carboxylation/diffusion balance in the mesophyll. In this case, delta(13)C values should become more negative as phosphoenolpyruvate carboxylase levels increase, unless there are offsetting changes in stomatal aperture. The principal effect of ribulose bisphosphate carboxylase/oxygenase on delta(13)C should occur through its effect on the extent of leakage of CO(2) from the bundle sheath cells. In this case, delta(13)C values should become more positive as ribulose bisphosphate carboxylase levels increase. Accordingly, the variation in delta(13)C values seen in maize leaves appears to be the result of variations in the level of phosphoenolpyruvate carboxylase.