Procedures for the Generation of Mature Chlamydomonas reinhardtii Zygotes for Molecular and Biochemical Analyses | Zendy

Dorothee Wegener | Zendy; Ulrike Treier | Zendy; Christoph F. Beck | Zendy

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Procedures for the Generation of Mature Chlamydomonas reinhardtii Zygotes for Molecular and Biochemical Analyses

Author(s) -

Dorothee Wegener,

Ulrike Treier,

Christoph F. Beck

Publication year - 1989

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.90.2.512

Subject(s) - zygote , chlamydomonas reinhardtii , chlamydomonas , gamete , biology , microbiology and biotechnology , botany , embryo , genetics , gene , embryogenesis , sperm , mutant

Zygotes represent an important stage in the sexual cycle of the unicellular green alga Chlamydomonas reinhardtii. To study zygote germination at a molecular level, a protocol was elaborated for the generation of zygotes in large quantities and a method was developed for the extraction from zygotes of RNA that could be translated in vitro.

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