Leghemoglobin in Lupin Plants (Lupinus albus cv Multolupa) | Zendy

Amparo Vivó | Zendy; José M. Andreu | Zendy; Sonsoles de la Viña | Zendy; María Rosario de Felipe | Zendy

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Leghemoglobin in Lupin Plants (Lupinus albus cv Multolupa)

Author(s) -

Amparo Vivó,

José M. Andreu,

Sonsoles de la Viña,

María Rosario de Felipe

Publication year - 1989

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.90.2.452

Subject(s) - leghemoglobin , lupinus , biology , glutaraldehyde , botany , araldite , chemistry , nitrogen fixation , root nodule , chromatography , genetics , adhesive , organic chemistry , layer (electronics) , bacteria

Leghemoglobin was localized by immunogold techniques in nodules of Lupinus albus cv Multolupa inoculated with Bradyrhizobium sp. (Lupinus) strain ISLU 16. The protein localization was performed in nodules embedded in Spurr's and Araldite epoxy resins and Lowycryl K4M. A very good preservation of both the ultrastructure and antigenicity was obtained with the tissues embedded in Araldite following glutaraldehyde fixation and unpostfixed in osmium tetroxide. Lupin leghemoglobin is a stable and abundant protein which allows a conventional method to be safely used for localization of leghemoglobin. Labeling of leghemoglobin was specifically confined to the cytosol matrix and nuclei. Gold particles were never observed in the peribacteroidal spaces nor in the cytoplasmic organelles of the infected cells. Decrease of leghemoglobin was observed when the plants were grown with 10.7 micromolar and 21.4 micromolar of nitrate.

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