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Identification of Gibberellins A1, A3, and Iso-A3 in Cultures of Azospirillum lipoferum
Author(s) -
Rubén Bottini,
Mónica Fulchieri,
David Pearce,
Richard P. Pharis
Publication year - 1989
Publication title -
plant physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.554
H-Index - 312
eISSN - 1532-2548
pISSN - 0032-0889
DOI - 10.1104/pp.90.1.45
Subject(s) - gibberellin , microbial inoculant , bioassay , gas chromatography , chromatography , mass spectrometry , chemistry , strain (injury) , biology , inoculation , botany , horticulture , genetics , anatomy
Gibberellins A(1), A(3), and iso-A(3) were identified from aseptic cultures of Azospirillum lipoferum strain op 33 by capillary gas chromatography-mass spectrometry (GC-MS) and GC-MS-selected ion monitoring. There were 20 to 40 picograms (in GA(3) equivalents, estimated from bioassay) of gibberellins A(1) and A(3) per milliliter of cell culture (containing 10(9) cells).

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