Effects of Singlet Oxygen Quenchers and pH on the Bacterially Induced Hypersensitive Reaction in Tobacco Suspension Cell Cultures | Zendy

Janet Salzwedel | Zendy; Margaret E. Daub | Zendy; JengSheng Huang | Zendy

Open Access

Effects of Singlet Oxygen Quenchers and pH on the Bacterially Induced Hypersensitive Reaction in Tobacco Suspension Cell Cultures

Author(s) -

Janet Salzwedel,

Margaret E. Daub,

JengSheng Huang

Publication year - 1989

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.90.1.25

Subject(s) - dabco , singlet oxygen , nicotiana tabacum , pseudomonas syringae , photochemistry , oxygen , chemistry , programmed cell death , hypersensitive response , cell , octane , cell culture , biophysics , biochemistry , biology , apoptosis , organic chemistry , gene , genetics

Suspension cell cultures of Nicotiana tabacum L. inoculated with the incompatible pathogen Pseudomonas syringae pv pisi undergo a hypersensitive reaction. Addition of the singlet oxygen quencher bixin to cell suspensions had no effect on hypersensitive cell death. Addition of the singlet oxygen quencher 1,4-diazabicyclo octane (DABCO) increased the medium pH and delayed the onset of cell death. This delay was eliminated when cell suspensions were buffered, and could also be induced by increasing medium pH with KOH. Bixin and DABCO also did not suppress the hypersensitive reaction in tobacco leaves. These data do not support a role for singlet oxygen in the hypersensitive reaction. Medium pH, however, appears to be a critical factor in cell suspension cultures.

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