Immunocytolocalization of Polygalacturonase in Ripening Tomato Fruit | Zendy

Denise M. Tieman | Zendy; Avtar K. Handa | Zendy

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Immunocytolocalization of Polygalacturonase in Ripening Tomato Fruit

Author(s) -

Denise M. Tieman,

Avtar K. Handa

Publication year - 1989

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.90.1.17

Subject(s) - ripening , lycopersicon , pectinase , horticulture , biology , botany , chemistry , enzyme , biochemistry

Using tissue blotting and immunocytolocalization, we have investigated the appearance and accumulation of polygalacturonase (PG) during tomato (Lycopersicon esculentum Mill.) fruit ripening. Results show that PG first appears in the collumella region followed by sequential appearance in the exopericarp and endopericarp, respectively. Detectable levels of PG were not present in the locular material containing seeds. This result indicates that PG synthesis initiates at the central collumella region of tomato fruit during ripening.

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