Open AccessImproved Method for HPLC Analysis of Polyamines, Agmatine and Aromatic Monoamines in Plant Tissue
Author(s) -
Robert D. Slocum,
Hector E. Flores,
Arthur W. Galston,
Leonard H. Weinstein
Publication year - 1989
Publication title -
plant physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.554
H-Index - 312
eISSN - 1532-2548
pISSN - 0032-0889
DOI - 10.1104/pp.89.2.512
Subject(s) - agmatine , putrescine , high performance liquid chromatography , derivatization , chromatography , polyamine , monoamine neurotransmitter , chemistry , nicotiana tabacum , cadaverine , tyramine , biogenic amine , quantitative analysis (chemistry) , plant tissue , amine gas treating , biochemistry , biology , botany , organic chemistry , enzyme , neurotransmitter , receptor , serotonin , gene
The high performance liquid chromatographic (HPLC) method of Flores and Galston (1982 Plant Physiol 69: 701) for the separation and quantitation of benzoylated polyamines in plant tissues has been widely adopted by other workers. However, due to previously unrecognized problems associated with the derivatization of agmatine, this important intermediate in plant polyamine metabolism cannot be quantitated using this method. Also, two polyamines, putrescine and diaminopropane, also are not well resolved using this method. A simple modification of the original HPLC procedure greatly improves the separation and quantitation of these amines, and further allows the simulation analysis of phenethylamine and tyramine, which are major monoamine constituents of tobacco and other plant tissues. We have used this modified HPLC method to characterize amine titers in suspension cultured carrot (Daucas carota L.) cells and tobacco (Nicotiana tabacum L.) leaf tissues.
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