Stabilization of Isolated Photosystem II Reaction Center Complex in the Dark and in the Light Using Polyethylene Glycol and an Oxygen-Scrubbing System | Zendy

Hugh McTavish | Zendy; Rafael Picorel | Zendy; M. Seibert | Zendy

Open Access

Stabilization of Isolated Photosystem II Reaction Center Complex in the Dark and in the Light Using Polyethylene Glycol and an Oxygen-Scrubbing System

Author(s) -

Hugh McTavish,

Rafael Picorel,

M. Seibert

Publication year - 1989

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.89.2.452

Subject(s) - photosystem ii , photosynthetic reaction centre , polyethylene glycol , photochemistry , chemistry , oxygen , data scrubbing , photosystem i , polyethylene , photosynthesis , organic chemistry , electron transfer , biochemistry , statistics , mathematics

The photosystem II reaction center as isolated (O Nanba, K Satoh [1987] Proc Natl Acad Sci USA 84: 109-112) is quite dilute and very unstable. Precipitating the complex with polyethylene glycol and resuspending it in buffer without detergent concentrates the reaction center and greatly improves its stability at 4 degrees C in the dark as judged by light-induced electron transport activity. Furthermore, a procedure was developed to minimize photodestruction of polyethylene-glycol-concentrated material at room temperature in the light. The ability to stabilize the photosystem II reaction center should facilitate future photophysical, biochemical, and structural studies of the complex.

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