Immunogold Localization of Photosynthetic Fructose-1,6-Bisphosphatase in Pea Leaf Tissue | Zendy

R. Hermoso | Zendy; Ma Rosario de Felipe | Zendy; Antonio De Vivo | Zendy; Ana Chueca | Zendy; Juan J. Lázaro | Zendy; Julio López Gorgé | Zendy

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Immunogold Localization of Photosynthetic Fructose-1,6-Bisphosphatase in Pea Leaf Tissue

Author(s) -

R. Hermoso,

Ma Rosario de Felipe,

Antonio De Vivo,

Ana Chueca,

Juan J. Lázaro,

Julio López Gorgé

Publication year - 1989

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.89.1.381

Subject(s) - fructose 1,6 bisphosphatase , immunogold labelling , chloroplast , pisum , biochemistry , photosynthesis , cytoplasm , cytosol , biology , enzyme , fructose , biophysics , colloidal gold , chemistry , botany , ultrastructure , nanotechnology , nanoparticle , materials science , gene

An enriched IgG serum fraction obtained from rabbits immunized against pea chloroplast fructose-1,6-bisphosphatase (FBPase) was used, coupled to colloidal gold (15 nanometer particles) goat anti-rabbit IgG, to analyze by electron microscopy the location of photosynthetic FBPase in pea (Pisum sativum L.) leaf ultrathin sections. In accordance with earlier biochemical studies on distribution of FBPase activity, the enzyme was visualized both in the stromal space and bound to the chloroplast membranes. Some gold particles also appear in the cytoplasm, which can be related to the presence in the cytosol of a high molecular weight precursor of this nuclear coded enzyme.

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