Preparation of Chlamydomonas Chloroplasts for the in Vitro Import of Polypeptide Precursors | Zendy

Michel GoldschmidtClermont | Zendy; Pia Malnoë | Zendy; J.-D. Rochaix | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Preparation of Chlamydomonas Chloroplasts for the in Vitro Import of Polypeptide Precursors

Author(s) -

Michel GoldschmidtClermont,

Pia Malnoë,

J.-D. Rochaix

Publication year - 1989

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.89.1.15

Subject(s) - chloroplast , chlamydomonas , chlamydomonas reinhardtii , rubisco , pyrenoid , in vitro , biochemistry , protein subunit , pyruvate carboxylase , biology , chemistry , botany , photosynthesis , enzyme , gene , mutant

To study the import of polypeptide precursors we have adapted and compared two procedures for the isolation of competent chloroplasts from the green unicellular alga, Chlamydomonas reinhardtii: silicasol gradient centrifugation and elutriation. The chloroplasts actively import the precursor of the small subunit of ribulose bisphosphate carboxylase-oxygenase in vitro.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research