In Vitro Synthesis, Assembly and Function of a Photosynthetic Membrane Protein | Zendy

Allan Michaels | Zendy; David L. Herrin | Zendy

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In Vitro Synthesis, Assembly and Function of a Photosynthetic Membrane Protein

Author(s) -

Allan Michaels,

David L. Herrin

Publication year - 1989

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.89.1.100

Subject(s) - membrane , chlamydomonas reinhardtii , chloroplast , biochemistry , in vitro , biology , chlamydomonas , translation (biology) , photosynthesis , membrane protein , biophysics , photoinhibition , microbiology and biotechnology , chemistry , photosystem ii , messenger rna , mutant , gene

Cell-free translation of Chlamydomonas reinhardtii RNA in the presence of photosynthetic membranes resulted in association of the herbicide binding (Qb) protein with membranes. Incubation of recovered membranes with high salt did not extract the polypeptide from membranes. Tryptic digestion of in vivo labeled membranes or membranes recovered from in vitro translation mixtures showed that Qb had similar orientation. In vitro translation in the presence of chloroplast membranes from cells exposed to high light intensity restored the membrane associated kinase activity lost by photoinhibition. Thus, in vitro synthesis resulted in functional integration of the Qb protein within the photosynthetic membrane.

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