Monoclonal Antibodies to 13-Deoxy-Gibberellins | Zendy

John Knox | Zendy; Michael H. Beale | Zendy; Geoffrey W. Butcher | Zendy; Jake MacMillan | Zendy

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Monoclonal Antibodies to 13-Deoxy-Gibberellins

Author(s) -

John Knox,

Michael H. Beale,

Geoffrey W. Butcher,

Jake MacMillan

Publication year - 1988

Publication title -

plant physiology

Language(s) - Uncategorized

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.88.4.959

Subject(s) - monoclonal antibody , keyhole limpet hemocyanin , gibberellin , radioimmunoassay , chemistry , antibody , conjugate , microbiology and biotechnology , biology , biochemistry , botany , immunology , mathematical analysis , mathematics

The production and characterization of two high affinity rat monoclonal antibodies to 13-deoxy-gibberellins is described. Hybrid myelomas were derived from rats immunized with an immunogenic keyhole limpet hemocyanin-gibberellin conjugate, linked at carbon-3 to gibberellin A(4) via a hemisuccinate bridge. The selected monoclonal antibodies were characterized by a competitive radioimmunoassay.

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