Hormonal Regulation of α-Amylase Expression in Barley Aleurone Layers

The expression of alpha-amylase isozymes in barley (Hordeum vulgare L.) aleurone layers is known to be maximally induced between 12 and 20 hours after addition of the phytohormone, gibberellic acid (GA(3)). Addition of another hormone, abscisic acid (ABA), or its metabolite, phaseic acid, during this time period resulted in reduced alpha-amylase expression. Expression of the high isoelectric point alpha-amylase isozyme group was affected much more by both of these treatments than was expression of the low isoelectric point alpha-amylase isozyme group. Addition of either the translation inhibitor cycloheximide or the transcription inhibitor cordycepin prevented the decrease in alpha-amylase mRNA levels after ABA treatment. Cordycepin also prevented the decreases in alpha-amylase expression that result from phaseic acid treatment. Midcourse GA(3) removal experiments were performed to determine whether ABA treatment and the removal of GA(3) have analogous effects on alpha-amylase expression. It was found that cordycepin treatment also prevented decreases in alpha-amylase mRNA levels resulting from GA(3) removal. We conclude that the suppression of alpha-amylase expression caused by ABA or midcourse GA(3) removal is dependent on continuous RNA and protein synthesis.