High Performance Liquid Chromatography-Based Reevaluation of Disaccharides Produced upon Incubation of Sugarcane Vacuoles with UDP-Glucose
Author(s) -
Andrew Maretzki,
Margaret Thom
Publication year - 1988
Publication title -
plant physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.554
H-Index - 312
eISSN - 1532-2548
pISSN - 0032-0889
DOI - 10.1104/pp.88.2.266
Subject(s) - sucrose , incubation , vacuole , chemistry , chromatography , ethanol , disaccharide , high performance liquid chromatography , uridine , sugar , saccharum , incubation period , elution , biochemistry , biology , botany , rna , cytoplasm , gene
A reanalysis of products formed after short-term incubation of sugarcane (Saccharum spp. hybrid cv H50-7209) vacuole preparations with uridine diphosphate [(14)C]glucose was performed. The results indicated that the ethanol-soluble substance previously identified as sucrose did not elute with sucrose when subjected to high performance liquid chromatography but had the same retention time as a disaccharide tentatively identified as laminaribiose.
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