Tonoplast Stability and Survival of Isolated Vacuoles in Different Buffers | Zendy

José León | Zendy; Jaleh Daie | Zendy; Rogér E. Wyse | Zendy

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Tonoplast Stability and Survival of Isolated Vacuoles in Different Buffers

Author(s) -

José León,

Jaleh Daie,

Rogér E. Wyse

Publication year - 1988

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.88.2.251

Subject(s) - vacuole , sucrose , hordeum vulgare , biology , biophysics , chemistry , biochemistry , botany , poaceae , cytoplasm

The mechanism of sucrose transport into vacuoles isolated from leaf tissue has been studied only in barley (Hordeum vulgare) mesophyll cells. In this tissue, sucrose transport was reported to be a facilitated diffusion. We have observed a facilitated diffusion of sucrose into vacuoles isolated from this tissue. However, no pH dependence was observed. Evidence is presented indicating that the pH dependence of sucrose uptake into vacuoles may be an artifact, reflecting tonoplast instability and survival of isolated vacuoles in different buffers. Apparently vacuoles do not withstand exposure to some commonly used buffers.

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