23Na-NMR Studies of the Intracellular Sodium Ion Concentration in the Halotolerant Alga Dunaliella salina | Zendy

Michal Bental | Zendy; Hadassa Degani | Zendy; Mordhay Avron | Zendy

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²³Na-NMR Studies of the Intracellular Sodium Ion Concentration in the Halotolerant Alga Dunaliella salina

Author(s) -

Michal Bental,

Hadassa Degani,

Mordhay Avron

Publication year - 1988

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.87.4.813

Subject(s) - dunaliella salina , intracellular , halotolerance , sodium , glycerol , molar concentration , extracellular , chemistry , nuclear magnetic resonance spectroscopy , nuclear chemistry , salinity , chromatography , biochemistry , biology , stereochemistry , botany , algae , organic chemistry , ecology

The Intracellular Na(+) concentration in the halotolerant alga Dunaliella salina was measured in intact cells by (23)Na-NMR spectroscopy, utilizing the dysprosium tripolyphosphate complex as a sodium shift reagent, and was found to be 88 +/- 28 millimolar. Intracellular sodium ion content and intracellular volume were the same, within the experimental error, in cells adapted to grow in media containing between 0.1 and 4.0 molar NaCl. These values assume extracellular and intracellular NMR visibilities of the (23)Na nuclei of 100 and 40%, respectively. The relaxation rate of intracellular sodium was enhanced with increasing salinity of the growth medium, in parallel to the intracellular osmosity due to the presence of glycerol, indicating that Na(+) ions and glycerol are codistribbuted within the cell volume.

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